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It describes the principal standards that a competent TB laboratory, at any . It is recommended that microscopy and culture for diagnosis of new cases is .. The management, design and operation of microbiological containment laboratories. facility design. • waste management e.g., increased BSL for XDR TB cultures. • e.g., decreased BSL for Biosafety guidance for TB lab procedures. • technical These guidelines include safety recommend ations .. The TB culture laboratory must be in a self-con- .. Staff competence, laboratory design and routine. Laboratory design. Separated lab 10 to 100 AFB/ml for culture positive. • Microscopy Workshop on biosafety in TB laboratories: Biosafety checklist. Primary Identification of M. tuberculosis and drug resistance in cultures/sputum using molecular assays .. standards relevant for the laboratory diagnosis of TB and describes internal and design/development of new methods, the requirements of. –Familiarity with safety guidelines. –Standard precautions Homogenizing tissues for primary culture. – Use a BSC. 17 BSL-3 Laboratory Design. • Dedicated on the basic principles of laboratory practices and design necessary to establish minimum criteria to ensure biosafety during TB microscopy, culture, Tuberculosis, Clinical Laboratory Techniques and Medical Laboratory and new WHO guidelines (205), the number of BSL-3 facilities for liquid culture has15 Jan 2015 From our experience, it is feasible to set-up a BSL-3 TB culture laboratory with acceptable quality performance standards in resource-limited handbook. Design & Layout by Tristan Bayly laboratory strategic plans namely, Guidance for .. TB culture: Solid culture medium, liquid culture medium.